이비인후과 의사와 상의하여 갑상선기능검사 및 조직검사등을 시행하여야 있다.
이학적검사를 시행한 후 경부 초음파검사와 갑상선기능검사를 시행하여 먼저 양성과 악성종물을 감별한다.
혈액검사를 통한 갑상선 기능검사와 핵의학과에서 시행하는 갑상선 주사검사를 시행하여 생물학적 기능을 평가할 수 있고 미세침흡인생검으로 최소한 3회 이상 반복 검사하여 진단이 내려질 때까지 검사한다.
역시 컴퓨터단층촬영(CT)과 자기공명영상소견이 종양의 위치와 크기를 판단하는데 도움을 줄 수 있다.
특징적으로 진단적 목적의 갑상선 호르몬억제요법을 시행하기도 하는데 악성결절은 크기의 감소가 없을 때 최고 40%까지 의심할 수 있다.
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